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rabbit polyclonal anti phospho raf 1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit polyclonal anti phospho raf 1
    Rabbit Polyclonal Anti Phospho Raf 1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+phospho+raf+1/Phospho-A-Raf+(Ser299)+Antibody/10__3390_slash_immuno6010005-123-47-51
    Average 93 stars, based on 20 article reviews
    rabbit polyclonal anti phospho raf 1 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Nanomodified Nexavar Enhances Efficacy in Caco-2 Cells via Targeting Aspartate β-Hydroxylase-Driven Mitochondrial Cell Death
    Article Snippet: .. To permeabilize the cells, they were resuspended in PBS with 0.1% Triton-X-100 for 3 min. For primary antibody staining, the cells were incubated overnight at 4 ◦C in PBS with 1% BSA, using the following primary antibodies: mouse monoclonal anti-Ras (H26, ab13833, Abcam, San Antonio, TX, USA), rabbit polyclonal anti-phospho-Raf-1 (Ser299, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-NF-κB (12741, Cell Signaling Technology, Danvers, MA, USA), and mouse monoclonal anti-phospho-ERK (7E10, Santa Cruz, Paso Robles, CA, USA), mouse monoclonal anti-Bax (NHP-AB171, Creative Biolabs, Frankfort, Germany), and rabbit polyclonal anti-ASPH (ab172475, Abcam, San Antonio, TX, USA) all diluted appropriately (1:5000). .. Following washing, the cells were incubated with secondary antibodies: goat anti-rabbit IgG (Alexa Fluor 488, ab150077, Abcam, San Antonio, TX, USA) or goat anti-mouse IgG (Alexa Fluor 594, ab150116, Abcam, San Antonio, TX, USA), each diluted 1:1000 in PBS with 1% BSA.

    Article Title: Inhibition of hepatitis C virus replication in HepG2 cells via modulation of the Raf-1 and interferon-alpha signaling pathways by thymoquinone.
    Article Snippet: liver hepatocytes [3].. The HCV virion has a lipid membrane envelope and measures 55 to 65 nm in diameter [4].. Its genome consists of a single-stranded positive-sense RNA, approximately 9.6 kb in length [5].

    Incubation:

    Article Title: Nanomodified Nexavar Enhances Efficacy in Caco-2 Cells via Targeting Aspartate β-Hydroxylase-Driven Mitochondrial Cell Death
    Article Snippet: .. To permeabilize the cells, they were resuspended in PBS with 0.1% Triton-X-100 for 3 min. For primary antibody staining, the cells were incubated overnight at 4 ◦C in PBS with 1% BSA, using the following primary antibodies: mouse monoclonal anti-Ras (H26, ab13833, Abcam, San Antonio, TX, USA), rabbit polyclonal anti-phospho-Raf-1 (Ser299, Cell Signaling Technology, Danvers, MA, USA), rabbit polyclonal anti-NF-κB (12741, Cell Signaling Technology, Danvers, MA, USA), and mouse monoclonal anti-phospho-ERK (7E10, Santa Cruz, Paso Robles, CA, USA), mouse monoclonal anti-Bax (NHP-AB171, Creative Biolabs, Frankfort, Germany), and rabbit polyclonal anti-ASPH (ab172475, Abcam, San Antonio, TX, USA) all diluted appropriately (1:5000). .. Following washing, the cells were incubated with secondary antibodies: goat anti-rabbit IgG (Alexa Fluor 488, ab150077, Abcam, San Antonio, TX, USA) or goat anti-mouse IgG (Alexa Fluor 594, ab150116, Abcam, San Antonio, TX, USA), each diluted 1:1000 in PBS with 1% BSA.

    Article Title: Inhibition of hepatitis C virus replication in HepG2 cells via modulation of the Raf-1 and interferon-alpha signaling pathways by thymoquinone.
    Article Snippet: liver hepatocytes [3].. The HCV virion has a lipid membrane envelope and measures 55 to 65 nm in diameter [4].. Its genome consists of a single-stranded positive-sense RNA, approximately 9.6 kb in length [5].



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